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产品名称： |
小型垂直电泳系统 |
| 产品编号： |
N2009717174510 |
| 发布日期： |
2009/7/29 |
| 查看次数： |
2132 |
| 产 品 详 细 介 绍 |
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Mini-PROTEAN 4 电泳系统
Components of the Mini-PROTEAN Tetra system. A, electrophoresis module, companion running module, tank, lid, and buffer dam; B, glass plates and combs*; C, sample loading guides**; D, casting stand and casting frames***.
The Mini-PROTEAN Tetra system is available complete with combs, plates, and casting accessories for 10-well, 0.75 mm and 1.0 mm thick gels (catalog #165-8000 and 165-8001, respectively). For other electrophoresis cell configurations, purchase the Mini-PROTEAN Tetra cell (catalog #165-8004) and a casting module with the appropriate well type and thickness.
For Ready Gel precast gel applications, order only the Mini-PROTEAN Tetra cell (165-8004).
Accessories and Replacement Parts
Combs — A variety of comb styles are available, including 5, 9, 10, and 15-well formats. The prep/2-D comb contains a reference well, and the IPG comb is designed to accommodate a 7 cm ReadyStrip IPG strip. These combs fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.
Plates for Hand Casting — To hand cast a gel, you need a short plate and a spacer plate. You do not need to order spacer separately; simply select the spacer plate that has the spacer width you need. (Spacer plates contain spacers already joined to the plate.) The short plates and the spacer plates fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.
Replacement Parts — Replacement parts are available for the Mini-PROTEAN Tetra cell, should you ever need them. Also available are casting accessories and sample loading guides.
配件定购信息
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目录 # |
描述 |
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Combs |
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165-3352 |
Mini-PROTEAN Comb, 5-well, 0.75 mm |
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165-3353 |
Mini-PROTEAN Comb, 9-well, 0.75 mm |
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165-3354 |
Mini-PROTEAN Comb, 10-well, 0.75 mm |
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165-3355 |
Mini-PROTEAN Comb, 15-well, 0.75 mm |
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165-3356 |
Mini-PROTEAN Comb, prep/2-D well, 0.75 mm |
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165-3357 |
Mini-PROTEAN Comb, 5-well, 1.0 mm |
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165-3358 |
Mini-PROTEAN Comb, 9-well, 1.0 mm |
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165-3359 |
Mini-PROTEAN Comb, 10-well, 1.0 mm |
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165-3360 |
Mini-PROTEAN Comb, 15-well, 1.0 mm |
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165-3361 |
Mini-PROTEAN Comb, prep/2-D well, 1.0 mm |
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165-3362 |
Mini-PROTEAN Comb, IPG well, 1.0 mm |
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165-3363 |
Mini-PROTEAN Comb, 5-well, 1.5 mm |
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165-3364 |
Mini-PROTEAN Comb, 9-well, 1.5 mm |
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165-3365 |
Mini-PROTEAN Comb, 10-well, 1.5 mm |
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165-3366 |
Mini-PROTEAN Comb, 15-well, 1.5 mm |
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165-3367 |
Mini-PROTEAN Comb, prep/2-D well, 1.5 mm |
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165-3368 |
Mini-PROTEAN Comb, IPG well, 1.5 mm |
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Plates for Hand Casting |
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165-3308 |
Short Plates, 5 |
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165-3310 |
Spacer Plates With 0.75 mm Integrated Spacers, 5 |
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165-3311 |
Spacer Plates With 1.0 mm Integrated Spacers, 5 |
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165-3312 |
Spacer Plates With 1.5 mm Integrated Spacers, 5 |
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Other Accessories |
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165-3313 |
Mini-PROTEAN 3 Casting Stand, with clamps and short plates |
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165-3130 |
Mini Cell Buffer Dams, 2 |
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165-3132 |
Sample Loading Guide, 15-well (blue) |
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165-3146 |
Sample Loading Guide, 10-well (yellow) |
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165-3201 |
Sample Loading Guide, 9-well (red) |
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165-3203 |
Sample Loading Guide, 12-well (green) |
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165-3303 |
Mini-PROTEAN Casting Stand |
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165-3304 |
Mini-PROTEAN Casting Frame |
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165-3305 |
Mini-PROTEAN Casting Stand Gaskets, replacement, 2 |
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165-3306 |
Mini-PROTEAN 3 Clamping Frame, 1 |
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165-3307 |
Mini-PROTEAN 3 Electrode Assembly, 1 |
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165-3320 |
Gel Releasers, 5 |
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165-4131 |
AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions |
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165-4132 |
Replacement Clamps, 2 |
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165-5131 |
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Ready Gel mini gels; includes instructions |
Mini-PROTEAN 3 电泳系统
序列号：165-3301
【介绍】
功能灵活的Mini–PROTEAN 3 电泳槽既可使用 Ready Gel 预制胶，也可使用手灌胶。 它是目前开发的相关产品中最方便的手灌胶操作系 统。Mini–PROTEAN 3 电泳系统省去了冗长的组装程 序，简化了灌胶和电泳过程。Mini–PROTEAN 3 电泳 槽可用于：
 一小时内完成小型胶电泳 一天内完成双向凝胶电泳 用于探索型的课题研究 筛选新样品 评估样品制备条件 制胶优点：
封边垫条永久地固定在长玻板上，保证玻板精确对齐，防止漏胶 凸轮卡锁的制胶框操作简单 *，在任何平面上都能精确对齐玻板 特殊的塑料电泳梳 *不会抑制凝胶聚合反应，制胶过程中，内置的脊可避免的空气接触，保证均一的凝胶聚合 标有厚度和孔数的玻板和电泳梳便于识别 平行排列的灌胶架*能同时看到正在灌制的两块凝胶，弹簧杠杆设计使得软橡胶衬垫产生良好的密封性 含封边垫条的长玻板加厚，使得玻板不宜破碎。 上样和电泳的创新性
专利的上样引导装置 **，防止泳道的遗漏上样或重复上样 电泳槽可同时进行2 块凝胶电泳，或使用塑模档板进行1 块凝胶电泳 模块式设计
模块式设计可使Mini–PROTEAN 3 电泳槽有多种用途。每个模块可与Mini–PROTEAN 3 的缓冲液槽和盖匹配， 形成一个完整的电泳槽。有以下4 个模块可选择：
Mini–PROTEAN 3 电泳槽，适合手灌胶或预制PAGE 和SDS–PAGE 胶 小型Trans–Blot 电泳槽，适合western 印迹杂交； 422 型电洗脱仪，用于电洗脱样品条带或整个泳道以进行探索研究； Mini–PROTEAN 2–D 电泳槽，用于双向应用中的第一向IEF 管式凝胶； 更多信息，请索取资料＃ 2378。
* US 专利6,162,342
** US 专利5,656,145
简易的装配流程。永久固定的封边垫条保证了胶板正确对位并防止漏胶。只需在任何平面上将制胶框和玻板组装起来，就完成了胶板对齐。制胶架上的弹簧杠杆可在橡胶密封衬垫上形成良好的密封性。
将凝胶夹（gel cassettes）装配好，转移到固夹框clamping frame（插入固夹框时，轻柔地把凝胶夹固定于适当位置），运用外表柔和的凸 轮卡锁将凝胶固定在密封垫上。这样凝胶就可以进行电泳了。
Mini–PROTEAN 3 电泳梳的每孔最大样品体积
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电泳梳厚度 |
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孔数或孔类型 |
孔宽度 |
0.5 mm |
0.75 mm |
1.0 mm |
1.5 mm |
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5 |
12.70 mm |
— |
70 µl |
105 µl |
160 µl |
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9 |
5.08 mm |
— |
33 µl |
44 µl |
66 µl |
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10 |
5.08 mm |
22 µl |
33 µl |
44 µl |
66 µl |
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15 |
3.35 mm |
13 µl |
20 µl |
26 µl |
40 µl |
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IPG |
76.20 mm |
— |
— |
420 µl |
730 µl |
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Prep/2–D |
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Reference well |
3.10 mm |
— |
13 µl |
17 µl |
30 µl |
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Sample well |
71.70 mm |
— |
310 µl |
400 µl |
680 µl |
将Mini–PROTEAN II 电泳槽升级到Mini–PROTEAN 3 电泳槽
只需3 个简单的步骤就可将Mini–PROTEAN II电泳槽改装成Mini–PROTEAN 3电泳槽：
1. 保留Mini–PROTEAN II 的槽和盖。
2. 定购Mini–PROTEAN II 升级组合套件（货号#165–3375）。
3. 定购所需的制胶模块。
选购适合您的电泳系统
可从预设配置中选择电泳系统，定购货号为#165–3301（10 孔、0.75 mm）或165–3322 （10 孔、1.0 mm）。 如要装配你自己的电泳系统，你可以购买Mini–PROTEAN 3 电泳模块（货号#165–3302）并从订货信息中另外选 择一种制胶模块。如果你只使用ReadyGel 预制胶，Mini–PROTEAN 3 电泳模块能满足你的需要。
【技术指标】
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凝胶数 |
1 或2 |
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预制胶 |
Ready Gel 预制胶 |
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手灌胶 |
Mini-PROTEAN 3 长玻板灌制 |
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玻板尺寸(W x L) |
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短板 |
10.1 x 7.3 cm |
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间隔板 |
10.1 x 8.2 cm |
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凝胶大小(W x L) |
手灌胶：8.3 x 7.3 cm; 预制胶： 8.6 x 6.8 cm |
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典型上层缓冲液体积 |
120 ml |
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典型下层缓冲液体积 |
180 ml |
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典型SDS-PAGE电泳时间 |
45 min ( 200 V 恒压) |
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建议电源 |
PowerPac Basic 或 PowerPac HC |
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大小(W x L x H) |
12 x 16 x 18 cm |
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重量 |
2.0 kg |
【订货信息】
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目录 # |
描述 |
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Mini-PROTEAN 3 Cell |
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165-3301 |
Mini-PROTEAN 3 Electrophoresis Cell, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam) |
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165-3302 |
Mini-PROTEAN 3 Electrophoresis Module, for Ready Gel precast gel applications, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam |
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Systems |
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165-2959 |
Mini-PROTEAN 3 Electrophoresis Cell/Mini Trans-Blot Electrophoretic Transfer Cell, 10-well, 0.75 mm thickness; complete system includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer chamber, lid with cables, instructions |
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165-3317 |
Mini-PROTEAN 3 Electrophoresis Cell and Mini Trans-Blot Module, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module |
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165-3318 |
Mini-PROTEAN 3 Electrophoresis Module and Mini Trans-Blot Module, includes electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam, Mini Trans-Blot module |
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165-3319 |
Mini-PROTEAN 3 Cell and PowerPac Basic Power Supply, 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), 100–120/220–240 V power supply includes power cord, instructions |
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165-3321 |
Mini-PROTEAN 3 Cell and Single-Row AnyGel Stand, includes 10-well, 0.75 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), single-row AnyGel stand holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions |
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165-3323 |
Mini-PROTEAN 3 Electrophoresis Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply |
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165-3324 |
Mini-PROTEAN 3 Electrophoresis Module, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 10-well, 1.0 mm thickness; complete system, includes 2 combs, 5 sets of glass plates, casting stand, casting clamp assembly, sample loading guide, electrophoresis module (electrode assembly, clamping frame, tank, lid with power cables, mini cell buffer dam), Mini Trans-Blot module, power supply |
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170-3989 |
Mini Trans-Blot Cell and PowerPac Basic Power Supply, includes 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer tank, lid with cables, power supply, power cord, instructions |
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Accessories |
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165-3320 |
Gel Releasers, 5 |
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165-4131 |
AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Ready Gel mini gels; includes instructions |
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165-4132 |
Replacement Clamps, 2 |
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165-5131 |
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 ReadyGel mini gels; includes instructions |
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Casting Modules |
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165-3325 |
Casting Module, 10-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3326 |
Casting Module, 15-well, 0.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3327 |
Casting Module, 5-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3328 |
Casting Module, 9-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3329 |
Casting Module, 10-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3330 |
Casting Module, 15-well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3331 |
Casting Module, prep/2-D well, 0.75 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates |
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165-3332 |
Casting Module, 5-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3333 |
Casting Module, 9-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3334 |
Casting Module, 10-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3335 |
Casting Module, 15-well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3336 |
Casting Module, prep/2-D well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates |
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165-3337 |
Casting Module, IPG well, 1.0 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates |
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165-3338 |
Casting Module, 5-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3339 |
Casting Module, 9-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3340 |
Casting Module, 10-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3341 |
Casting Module, 15-well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates, sample loading guide |
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165-3342 |
Casting Module, prep/2-D well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates |
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165-3343 |
Casting Module, IPG well, 1.5 mm, includes casting stand, 2 casting frames, 2 combs, 5 sets of plates |
Mini-PROTEAN 3 多板凝胶灌胶器
序列号：165-4110
【介绍】
使用Mini-PROTEAN 3多板凝胶灌胶器，你可同时灌制多达12 块厚度 为0.5、0.75、1.0 或1.5 mm 的凝胶。当灌制凝胶数少于12 块时，丙烯模 块可充当空间填充物。你可通过485型梯度生成仪从底部注入液体，灌制可重复性的梯度胶。
【技术指标】
【订货信息】
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目录 # |
描述 |
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Mini-PROTEAN 3 Multi-Casting Chamber |
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165-4110 |
Mini-PROTEAN 3 Multi-Casting Chamber, includes 8 acrylic blocks, 15 separation sheets, tapered luer connector, stopcock valve (order glass plates and combs separately) |
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165-4111 |
Mini-PROTEAN 3 Multi-Casting Chamber, with 0.75 mm spacer plates, includes 15 sets of glass plates (order combs separately) |
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165-4112 |
Mini-PROTEAN 3 Multi-Casting Chamber, with 1.0 mm spacer plates, includes 15 sets of glass plates (order combs separately) |
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165-4113 |
Mini-PROTEAN 3 Multi-Casting Chamber, with 1.5 mm spacer plates, includes 15 sets of glass plates (order combs separately) |
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165-4116 |
Mini-PROTEAN 3 Multi-Casting Chamber, with 0.5 mm spacer plates, includes 15 sets of glass plates (order combs separately) |
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Combs and Glass Plates for 2-D Electrophoresis |
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165-3308 |
Short Plates, 5 |
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165-3309 |
Spacer Plates With 0.5 mm Integrated Spacers, 5 |
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165-3310 |
Spacer Plates With 0.75 mm Integrated Spacers, 5 |
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165-3311 |
Spacer Plates With 1.0 mm Integrated Spacers, 5 |
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165-3312 |
Spacer Plates With 1.5 mm Integrated Spacers, 5 |
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165-3356 |
Mini-PROTEAN 3 Comb, prep/2-D well, 0.75 mm |
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165-3361 |
Mini-PROTEAN 3 Comb, prep/2-D well, 1.0 mm |
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165-3362 |
Mini-PROTEAN 3 Comb, IPG well, 1.0 mm |
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165-3367 |
Mini-PROTEAN 3 Comb, prep/2-D well, 1.5 mm |
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165-3368 |
Mini-PROTEAN 3 Comb, IPG well, 1.5 mm |
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Accessories |
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165-3320 |
Gel Releasers, 5 |
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165-4114 |
Acrylic Blocks, 6 mm, 8 |
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165-4115 |
Separation Sheets, 15 |
Mini-PROTEAN 3 Dodeca 小型高通量电泳槽
序列号：165-4100
【介绍】
Mini-PROTEAN 3 Dodedca 小型高通量电泳槽在完全相同的条件下，只需35 分钟就能进行12 块小型胶电泳。 运用Mini-PROTEAN 3 多板凝胶灌胶器和485 型梯度生成仪可一次灌制12 块凝胶，避免了凝胶间的 差异。也可以使用Ready Gel 预制胶，能提供高通量实验室所需的更多简便性和可重复性。Mini-PROTEAN 3 Dodedca小型高通量电泳槽有以下特点：
可同时进行12 块手灌胶或预制胶电泳 内含冷却管可外接冷却循环器，防止温度过高并保证结果的最佳分辨率 搅拌功能可维持缓冲液槽温度的一致性，确保电泳的可重复性 特别设计的固夹框（已申请专利）方便装配 内置的快速排水口可方便地将缓冲液排出 外形小巧，节省空间
【技术指标】
|
凝胶数量 |
1 － 12 块 |
|
预制胶 |
Ready Gel 预制胶 |
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手灌胶 |
使用Mini-PROTEAN 3 带封边垫条的长玻板和
Mini-PROTEAN 3 多板凝胶灌胶器灌制 |
|
凝胶大小(W x L) |
手灌：8.3 x 7.3 cm；预制：8.6 x 6.8 cm |
|
凝胶厚度 |
0.5、0.75、1.0 或1.5mm ( 预制胶只有1.0 mm 规格) |
|
典型电泳条件 |
200 V 恒压、600 mA、最大功率为120 W |
|
建议电源 |
PowerPac HC |
|
制冷 |
内置冷却管可外接冷却循环器（外接冷却循环器需另行购买；
推荐流速10-15L/min，推荐制冷功率>250 W，20°C） |
|
所需总缓冲液体积 |
3.4–4.4 L |
|
大小(W x L x H) |
16.2 x 41.5 x 15.0 cm |
|
重量 |
5 kg (11 lb) |
【订货信息】
|
目录 # |
描述 |
|
Mini-PROTEAN 3 Dodeca Cell |
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165-4100 |
Mini-PROTEAN 3 Dodeca Cell, includes tank with built-in cooling coil, lid with power cables, 6 electrophoresis clamping frames, 2 buffer dams, drain line, 2 gel releasers |
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165-4101 |
Mini-PROTEAN 3 Dodeca Cell With Multi-Casting Chamber, includes tank with built-in cooling coil, lid with power cables, 6 electrophoresis clamping frames, 2 buffer dams, drain line, 2 gel releasers, multi-casting chamber, 8 acrylic blocks, 15 separation sheets, tapered luer connector, stopcock valve |
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Replacement Parts |
|
165-4102 |
Replacement Electrophoresis Clamping Frame, 1 |
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165-4103 |
Lower Electrode Assembly With Platinum Wire |
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165-4104 |
Replacement Drain Line, 1 |
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165-4105 |
Replacement Cooling Coil, includes connector tubing |
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165-2948 |
Replacement Power Cables, for lid |
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Accessories |
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164-5052 |
PowerPac HC Power Supply, 100–120/220–240 V; includes power cord, instructions |
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165-3130 |
Mini Cell Buffer Dams, 2 |
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165-3320 |
Gel Releasers, 5 |
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165-4110 |
Mini-PROTEAN 3 Multi-Casting Chamber, includes 8 acrylic blocks, 15 separation sheets, tapered luer connector, stopcock valve (order glass plates and combs separately) |
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165-4120 |
Model 485 Gradient Former, 40–175 ml, includes body with valve stem and tubing connection kit | |
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